Flow Cytometry

High quality flow cytometry service to support your development from pre-clinical to clinical sample analysis

  • Tailored to your specific needs
  • Underpinned by extensive Tissue Culture Facility
  • Access to in-house panels
  • Experienced Principal Scientist team
  • Validated 21 CFR part 11 Compliant Acquisition & Analysis software
  • Collaboration with National Measurement Laboratory

Contact us to discuss flow cytometry 

Download our flow cytometry overview flyer

Principal Scientist, Teona Roschupkina, introduces our flow cytometry service and capabilities. 

Our flow cytometry service

With the growth of the biopharmaceuticals industry, the impact of biological drugs on the immune system has been a key area of investigation for pre-clinical and clinical drug development. Flow cytometry provides an ideal tool to examine this impact, allowing the interrogation of immunological changes at the level of a single cell to define drug related effects.

Our flow cytometry service offers a range of assays to support pre-clinical through to late phase drug development. With two 13-colour, Beckman Coulter CytoFLEX cytometers we are well equipped to meet your bioanalytical needs. Both instruments are fully validated to meet GLP and GCP regulatory guidelines.

Complex Assay Development

We run GxP regulated studies & provide full method development & validation services. Alongside our established PK, PD & immunogenicity testing, our flow cytometry service provides supplementary quantitation of immunological endpoints.

We have a breadth of experience in the development of complex cytometry end-points, in both human samples and a range of pre-clinical species, including:

  • Immunophenotyping
  • Receptor-ligand binding assays (receptor occupancy)
  • Intracellular cytokines
  • Phosphoflow
  • Cellular activation
  • nAbs
Image showing CytoFlex 2 instrument Beckman Coulter CytoFlex

Flow Cytometry Platform


  • Two Beckman Coulter CytoFLEX, 3 laser
  • Two configuration matched instruments
  • 13 Fluorescence, Standard FSC/SSC+ Violet SSC
  • Cross Standardized for each assay
  • Linearity, >99%
  • Fully Validated to current guidelines (2021)


  • Dedicated Cell Culture and handling
  • Cell washer for improved precision
  • Technical specialist team
  • FCS Express Fully validated to current guidelines
  • All assays are Aligned with current H62 guideline (published 2021)

Regulations in Flow Cytometry

For method development and validation, we follow the current industry standards for fit-for-purpose flow cytometry method validation and offer a full method development and validation service. At present, there are no formal regulatory guidelines available for the validation of Flow Cytometry assays.

Flow Cytometry - Sample Processing Packages

Flow cytometric measurement of protein phosphorylation is a critical pathway for understanding a drug’s mechanism of action. Correlating this data with other key parameters such as pharmacokinetics (PK), pharmacodynamics (PD), and immunogenicity provides deeper insights into the drug’s effectiveness on living cells.

However, the instability of living cell functions outside the body complicates logistical arrangements and increases the cost of performing such analyses.

Our Flow Cytometry team have designed a sample processing package that solves this problem.

The process involves:

  • Stabilising sample before shipping
  • Batch samples from one subject
  • Decrease variability

Learn more about the process below and contact us to discuss your project.